The emerging age of cell-free synthetic biology.

نویسندگان

  • Mark Thomas Smith
  • Kristen M Wilding
  • Jeremy M Hunt
  • Anthony M Bennett
  • Bradley C Bundy
چکیده

The engineering of and mastery over biological parts has catalyzed the emergence of synthetic biology. This field has grown exponentially in the past decade. As increasingly more applications of synthetic biology are pursued, more challenges are encountered, such as delivering genetic material into cells and optimizing genetic circuits in vivo. An in vitro or cell-free approach to synthetic biology simplifies and avoids many of the pitfalls of in vivo synthetic biology. In this review, we describe some of the innate features that make cell-free systems compelling platforms for synthetic biology and discuss emerging improvements of cell-free technologies. We also select and highlight recent and emerging applications of cell-free synthetic biology.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Ethical Debates on Synthetic Biology

Background: As an emerging interdisciplinary area of science with a multitude of potential facilities and applications, synthetic biology integrates different disciplines with one another ranging from basic science to engineering. This interdisciplinary branch therefore encompasses sciences that require specific development and ethical approaches. Materials and Methods: Science management princ...

متن کامل

Highly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System

Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...

متن کامل

Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression.

Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create a practical, reproducible, and a labor- and cost-effective approach for rapid production of bacterial...

متن کامل

Emerging insights into the biology of metastasis: A review article

Metastasis means the dissemination of the cancer cells from one organ to another which is not directly connected to the primary site. Metastasis has a crucial role in the prognosis of cancer patients. A few theories, different types of cell and several molecular pathways have been proposed to explain the mechanism of metastasis. In this work, the related articles in the limited period of time, ...

متن کامل

Synthetic Biology Based on Genetic Logic Circuit, Using the Expression of Drug Resistance, BCRP Pump in MCF-7 Cancer Cell Line

Biological circuits are developed as biological parts within a cell to carry out logical functions resembling those studied in electronics circuits. These circuits can be performed as a method to vary cellular functions, to develop cellular responses to environmental conditions, or to regulate cellular developments. This research explored the possibility of synthetic biology based on the geneti...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • FEBS letters

دوره 588 17  شماره 

صفحات  -

تاریخ انتشار 2014